Background: The study was aimed to investigate the productivity and fertility status of the crossbred and Watani (local) cows in Jalalabad and role of blood metabolites in reproductive cyclicity.
Materials and Methods: A total of 100 dairy cows were selected in five zones comprising city, north, west, south and east of Jalalabad. Fifty crossbred cows (French Friesian x Watani) and 50 Watani cows were selected. Blood samples were collected (10 ml) for blood metabolites and hormonal profiles on day 1, 4 and 17th of the estrus cycle and analysed through UV/Vis spectrophotometer and hormonal profiles through ELISA using Humareader.
Findings: The concentrate intake in crossbred cows was 2.00 ± 0.20 ranging from 1.63 to 2.42 kg day-1, while in Watani cows the values were 2.20 ± 0.21, 1.80 and 3.30 kg day-1, respectively. The milk production for crossbred cows was 7.64 ± 0.14 ranging from 7.28 to 8.00 kg day-1, while in Watani cows the values were 3.75 ± 0.49, 2.00 and 4.88 kg day-1, respectively. Mucus discharge in crossbred cows was 2.00 ± 0.22 (1.50 to 2.50 scales) against 2.45 ± 0.19 in Watani cows. Standing for mounting in crossbred cows was higher (0.43 ± 0.86) than Watani cows (0.26 ± 0.09; scale 0 to 4), Triglycerides varied from 100.94±2.79 to 117.52±5.77 mg/dl (P=0.024) with significant effect of estrus days but no effect of region. Serum glucose in crossbred cows was 67.18±1.50 mg/dl while in Watani cows, it was 65.20±1.33. Protein was 7.62±0.11 in crossbred cows and 6.99±0.13 g/dl in Watani ones. The respective values for triglycerides were 110.69±3.43 and 104.03±3.40 mg/dl. Progesterone concentrations were 0.581±0.076 ng/ml and 0.569±0.09 ng/ml while LH was 28.41±3.31 and 24.20±2.64 ng/ml in the two breeds. All the blood metabolites and hormonal concentrations were not different among regions, however triglycerides showed a constant increase during the succeeding phases while glucose manifested an opposite pattern during the estrus cycle. The level of glucose in the city animals was higher (69.21±2.37 mg/dl, (P=0.127). Progesterone concentration increased on day 4th and declined on 17th day while LH showed a consistent increase during the period.
Conclusion: This study concluded that breeding of the local Watani cows with French Frisian semen resulted in production of crossbred cows. The crossbred cows showed higher concentrations of blood glucose, triglycerides, proteins, progesterone and LH hormones, reflecting higher metabolic activities than the local Watani cows.
KeywordsBlood, Metabolite, Hormone, Dairy Cow, Estrus Cycle
- Adams, D. C., Short, R. E., & Knapp, B. W. (1987). Body size and body condition effects on performance and behavior of grazing beef cows. Nutrition reports international, 35(2), 269-277.
- Ahmad, I., Lodhi, L. A., Qureshi, Z. I., & Younis, M. (2004). Studies on blood glucose, total proteins, urea and cholesterol levels in cyclic, non-cyclic and endometritic crossbred cows. Pakistan Veterinary Journal, 24(2), 92-94.
- Antunović, Z., Senčić, Đ., Šperanda, M., & Liker, B. (2002). Influence of the season and the reproductive status of ewes on blood parameters. Small Ruminant Research, 45(1), 39-44.
- Anderson, A. K., Gayley, H. E., & Pratt, A. D. (1930). Studies on the chemical composition of bovine blood. Journal of Dairy Science, 13(4), 336-348.
- Chase Jr, C. C., Del Vecchio, R. P., Smith, S. B., & Randel, R. D. (1992). In vitro metabolism of glucose by bovine reproductive tissues obtained during the estrous cycle and after calving. Journal of animal science, 70(5), 1496-1508.
- Dutta, J. C., Baruah, R. N., Dutta, L., & Talukdar, S. C. (1988). Blood biochemical-studies in anestrous and normal cyclic cattle. Indian Veterinary Journal, 65(3), 239-241.
- El-Sherif, M. M. A., & Assad, F. (2001). Changes in some blood constituents of Barki ewes during pregnancy and lactation under semi-arid conditions. Small Ruminant Research, 40(3), 269-277.
- Gaikwad, S. M., Dhoble, R. L., Sawale, A. G., Mane, P. M., & Dawane, S. C. (2007). Osteomalacia in Holstein Friesian cow: a case report. Intas Polivet, 8(2).
- Husain, S. S., & Mostafa, K. G. (1985). Reproductive potentialities of Local and Crossbred animals under farm and village conditions. Livestock Adviser, 10, 15-19.
- Haruna, S., Kuroiwa, T., Lu, W., Zabuli, J., Tanaka, T., & Kamomae, H. (2009). The effects of short-term nutritional stimulus before and after the luteolysis on metabolic status, reproductive hormones and ovarian activity in goats. Journal of Reproduction and Development, 55(1), 39-44.
- Iriadam, M. (2007). Variation in certain hematological and biochemical parameters during the peri-partum period in Kilis does. Small Ruminant Research, 73(1-3), 54-57.
- Karapehlivan, M., Atakisi, E., Atakisi, O., Yucayurt, R., & Pancarci, S. M. (2007). Blood biochemical parameters during the lactation and dry period in Tuj ewes. Small Ruminant Research, 73(1-3), 267-271.
- Khan, M. K. I., & Khatun, M. J. (1998). Performances of F1 crossbred cows at Baghabarighat milk shed area. Bangladesh Journal of Animal Science, 27(1), 183-186.
- Leroy, J. L. M. R., Vanholder, T., Delanghe, J. R., Opsomer, G., Van Soom, A., Bols, P. E. J., & de Kruif, A. (2004). Metabolite and ionic composition of follicular fluid from different-sized follicles and their relationship to serum concentrations in dairy cows. Animal reproduction science, 80(3-4), 201-211.
- McClure, T. J., Nancarrow, C. D., & Radford, H. M. (1978). The Effect of 2-Deoxy-N-Glucose on Ovarian Function of Cattle. Australian Journal of Biological Sciences, 31(2), 183-186.
- Nazifi, S., Saeb, M., & Ghavami, S. M. (2002). Serum lipid profile in iranian fat‐tailed sheep in late pregnancy, at parturition and during the post‐parturition period. Journal of Veterinary Medicine Series A, 49(1), 9-12.
- Qureshi, M. S., Khan, S., & Ahmad, N. (2007). Pregnancy depresses milk yield in dairy buffaloes. Italian Journal of Animal Science, 6(sup2), 1290-1293.
- Qureshi, M. S., Habib, G., Samad, H. A., Siddiqui, M. M., Ahmad, N., & Syed, M. (2002). Reproduction-nutrition relationship in dairy buffaloes. I. Effect of intake of protein, energy and blood metabolites levels. Asian-australasian journal of animal sciences, 15(3), 330-339.
- Rutter, L. M., Randel, R. D., Schelling, G. T., & Forrest, D. W. (1983). Effect of abomasal infusion of propionate on the GnRH-induced luteinizing hormone release in prepuberal heifers. Journal of animal science, 56(5), 1167-1173.
- Rahman, M., Ahmed, N., & Ahmed, A. R. (1987). A comparative study on some productive and reproductive performances of dairy cows at Savar Dairy and Cattle Improvement Farm [Bangladesh]. Bangladesh Veterinary Journal (Bangladesh).
- Sarker, M. S., Prodhan, M. A. M., Huque, K. S., Islam, S. K. M. A., Asgar, M. A., & Kibria, A. S. M. G. (2011). Glucose and total protein of red Chittagong cattle and local cattle at Chittagong. Asian Journal of Biological Sciences, 4(3), 312-316.
- Sprague, E. A., Hopwood, M. L., Niswender, G. D., & Wiltbank, J. N. (1971). Progesterone and luteinizing hormone levels in peripheral blood of cycling beef cows. Journal of animal science, 33(1), 99-103.
- Silanikove, N. (2000). Effects of heat stress on the welfare of extensively managed domestic ruminants. Livestock production science, 67(1-2), 1-18.
- Schlumbohm, C., Sporleder, H. P., Gurtler, H., & Harmeyer, J. (1997). The influence of insulin on metabolism of glucose, free fatty acids and glycerol in normo-and hypocalcemic ewes during different reproductive states. Deutsche Tierarztliche Wochenschrift, 104(9), 359-365.