Isolation and Identification of Bovine Pasteurella Multocida Type A and Its Drug Sensitivity Analysis
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Abstract
Pasteurella multocida is a bacteria frequently associated with pneumonia in bovine respiratory disease. P. multocida was initially discovered in 1878 AC in cholera-infected poultry as causitive agetnt for serious infectious diseases such as avian cholera, bovine hemorrhagic septicemia, and porcine atrophic rhinitis. This study aimed to isolate and identify Pasteurella multocida type A, and conducted a drug sensitivity analysis. To identify P. multocida type A. 30 samples were collected and sent to a lab from farms and free-range farmers in Changchun, Yushu, Siping, Nong'an, Yanji, Baicheng, Dunhua, Baishan, and other areas of Jilin Province during 2020 and 2022. The study was designed to perform bacterial isolation and identification, preservation, and drug susceptibility testing. The PCR amplification procedure used 16S rDNA primers. The desired 1450 bp target bands were attained. The target bands were isolated, attached to the pMD18-T vector, and transformed into DH5-competent cells. Positive cells were then selected by screening. The clones were sequenced, and the resulting sequences were compared with those in GenBank using the BLAST algorithm. In the result, a total of Twelve P. multocida strains were discovered. The MIC responses of the examined strains to the 12 antibacterial medications showed notable variations. The maximum MIC values for gentamycin and kanamycin were 64 μg/mL and 32 μg/mL, respectively, for the tested strains of tilmicosin were ≤32 μg/mL. The MIC values for florfenicol and doxycycline appeared greater than 512 μg/mL. The MIC for ciprofloxacin and enrofloxacin is up to 16 μg/mL. There are significant disparities in the levels of antibiotic resistance among the tested strains. The drug resistance rates of the tested strains to several antibacterial medications were tallied, and tilmicosin had the highest drug resistance rate (68%), followed by terramycin (63%). The other strains displayed varied degrees of drug resistance, with resistance rates below 50%. However, sulfadiazine didn’t shown any resistance.
Keywords
Bovine, respiratory, disease, Drug, sensitivity, analysis, Identification, Isolation, P. multocida##plugins.themes.academic_pro.article.details##
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