Types of papers

Original research paper, Review paper, Systematic review, Mini review, and case report.  

Original research paper

For an original research paper, the manuscript should be arranged in the following order: Title page (Title, Full Author name, Author affiliate address & email of the corresponding author), Abstract, Keywords, Introduction, Materials, and methods, Results, Discussion, Acknowledgements, References, Tables with legends, Figures with legends and Supplementary materials (if applicable).

Manuscript Submission

Submission of a manuscript implies: that the work described has not been published before; that it is not under consideration for publication anywhere else; that its publication has been approved by all co-authors, if any, as well as by the responsible authorities – tacitly or explicitly – at the institute where the work has been carried out. The publisher will not be held legally responsible should there be any claims for compensation.


Authors wishing to include figures, tables, or text passages that have already been published elsewhere are required to obtain permission from the copyright owner(s) for both the print and online format and to include evidence that such permission has been granted when submitting their papers. Any material received without such evidence will be assumed to originate from the authors.

Review Procedure

Authors are expected to receive the reviewer’s comment within two weeks followed by editorial verification. Incomplete manuscripts and papers not written according to instruction will be returned to the author without sending to reviewers. The revised manuscript should be returned.


All manuscripts should be submitted to nuijb@nu.edu.af via e-mail attachment as a single Microsoft word file. Manuscripts that are not written according to journal instructions will be returned immediately. For online submission please follow the hyperlink “Submit a manuscript” and upload all of your files (Cover letter, copyright form, and single manuscript) following the instructions given on the screen.

Publication charge

The processing fee in the Nangarhar University International Journal of Biosciences (NUIJB) is payable after the article is accepted for publication. The processing fee for one article is USD 100 for international authors and USD 50 for Afghani authors.

Source Files

Please ensure you provide all relevant editable source files at every submission and revision. Failing to submit a complete set of editable source files will result in your article not being considered for review. For your manuscript text please always submit in common word processing formats such as .doc, docx.

Manuscript Preparation


The title should be concise and informative.

Author information

  • The name(s) of the author(s)
  • The affiliation(s) of the author(s), i.e. institution, (department), city, (state), country
  • A clear indication and an active e-mail address of the corresponding author
  • If available, the 16-digit ORCID of the author(s)

If the address information is provided with the affiliation(s) it will also be published.

For authors that are (temporarily) unaffiliated we will only capture their city and country of residence, not their e-mail address unless specifically requested.


Please provide an abstract of 250 to 300 words. The abstract should not contain any undefined abbreviations or unspecified references. The abstract should follow the style of structured abstracts, with headings: 1) Background: Place the question addressed in a broad context and highlight the purpose of the study; 2) Materials and Methods: Describe briefly the main methods or treatments applied. Include any relevant preregistration numbers, and species and strains of any animals used. 3) Findings: Summarize the article's main findings; and   4) Conclusion: Indicate the main conclusions or interpretations. The abstract should be an objective representation of the article: it must not contain results that are not presented and substantiated in the main text and should not exaggerate the main conclusions.


Please provide 4 to 6 keywords that can be used for indexing purposes.


The introduction should briefly place the study in a broad context and highlight why it is important. It should define the purpose of the work and its significance, including specific hypotheses being tested. The current state of the research field should be reviewed carefully and key publications cited. Please highlight controversial and diverging hypotheses when necessary. Finally, briefly mention the main aim of the work. Keep the introduction comprehensible to scientists working outside the topic of the paper.  

Materials and Methods

 They should be described with sufficient detail to allow others to replicate and build on published results. New methods and protocols should be described in detail while well-established methods can be briefly described and appropriately cited. Give the name and version of any software used and make clear whether the computer code used is available. Include any pre-registration codes.


Provide a concise and precise description of the experimental results, their interpretation as well as the experimental conclusions that can be drawn.


Authors should discuss the results and how they can be interpreted from the perspective of previous studies and the working hypotheses. The findings and their implications should be discussed in the broadest context possible and the limitations of the work highlighted. Future research directions may also be mentioned.


This section is mandatory, with one or two paragraphs to end the main text.


Acknowledgments of people, grants, funds, etc. should be placed in a separate section on the Manuscript. The names of funding organizations should be written in full.

Conflicts of Interest 

Authors must identify and declare any personal circumstances or interests that may be perceived as influencing the representation or interpretation of reported research results. If there is no conflict of interest, please state "The authors declare no conflict of interest." 

Text Formatting

Manuscripts should be submitted in Word Processing (.doc, .docx).

  • Use a normal, plain font (e.g., 10-point Times Roman) for text.
  • Manuscripts should be typewritten on an A4 sheet having ‘1.5’ line-spacing throughout the text. The margins should be 1inch on all sides and the page number should be consecutively on the bottom of the page.
  • Use italics for emphasis.
  • The manuscript should have a line number.
  • Use the automatic page numbering function to number the pages.
  • Do not use field functions.
  • Use tab stops or other commands for indents, not the space bar.
  • Use the table function, not spreadsheets, to make tables.
  • Use the equation editor or Math Type for equations.
  • Save your file in .docx or .doc format (Word Processing).

Preparing Figures and Tables


  • All tables are to be numbered using Arabic numerals.
  • Tables should always be cited in text in consecutive numerical order.
  • For each table, please supply a table caption (title) explaining the components of the table.
  • Identify any previously published material by giving the original source in the form of a reference at the end of the table caption.
  • Footnotes to tables should be indicated by superscript lower-case letters (or asterisks for significance values and other statistical data) and included beneath the table body.


  • All figures are to be numbered using Arabic numerals.
  • Figures should be submitted within the body of the text. Only if the file size of the manuscript causes problems in uploading it, the large figures should be submitted separately from the text.
  • When preparing your figures, size figures to fit in the column width.
  • Figures should always be cited in text in consecutive numerical order.
  • Figure parts should be denoted by lowercase letters (a, b, c, etc.).
  • If an appendix appears in your article and it contains one or more figures, continue the consecutive numbering of the main text. Do not number the appendix figures,"A1, A2, A3, etc.", however, be numbered separately.
  • Avoid effects such as shading, outline letters, etc.
  • Keep lettering consistently sized throughout your final-sized artwork, usually about 2–3 mm (8–12 pt).


Abbreviations should be defined at first mention and used consistently thereafter.

Scientific style

  • Please always use internationally accepted signs and symbols for units (SI units).
  • Genus and species names should be in italics.
  • Generic names for drugs and pesticides are preferred; if trade names are used, the generic name should be given at first mention.



Reference citations in the text should be identified by APA 6th edition. Some examples:

  1. This effect has been widely studied (Rahmani et al., 2022).
  2. Our study suggests that SeZnP supplementation serves as possible and best nutritive as ZnP or SeP for Wistar rats raised under high ambient temperature (Ali, 2020).
  3. The knowledge of the mechanisms through which the metabolites produced by the gut microbiota (postbiotics) prevent diseases (Ahmad & Rahim, 2021).

Reference list

The list of references should only include works that are cited in the text and that have been published or accepted for publication.

If available, please always include DOIs as full DOI links in your reference list (e.g. “https://doi.org/10.1016/j.lfs.2020.117464”).

Journal article

Malyar, R. M., Li, H., Liu, D., Abdulrahim, Y., Farid, R. A., Gan, F., . . . Huang, K. (2020). Selenium/Zinc-Enriched probiotics improve serum enzyme activity, antioxidant ability, inflammatory factors and related gene expression of Wistar rats inflated under heat stress. Life sciences, 248, 117464.

Ideally, the names of all authors should be provided, but the usage of “et al” in long author lists will also be accepted:

Article by DOI

Malyar, R. M., Li, H., Liu, D., Abdulrahim, Y., Farid, R. A., Gan, F., . . . Huang, K. (2020). Selenium/Zinc-Enriched probiotics improve serum enzyme activity, antioxidant ability, inflammatory factors, and related gene expression of Wistar rats inflated under heat stress. Life sciences, 248, 117464.https://doi.org/10.1016/j.lfs.2020.117464