In-vitro propagation of pre-pubertal bovine Spermatogonial Stem Cell in prepation for transplantation

##plugins.themes.academic_pro.article.sidebar##

Published Feb 25, 2023
https://doi.org/10.70436/nuijb.v2i01.17
Download
PDF 234
Statistic

Downloads

Download data is not yet available.
Vol. 2 No. 01 (2023): February

##plugins.themes.academic_pro.article.main##

Mohammad Kazim Wardak
Aziz Ur Rahman Khalid
Zabihullah Jameel

Abstract

‌Backgroud: We investigated the expression of stem cell markers in in -vitro cultivated speramatogonial stem cells (SSCs) with the intention of developing a useful system to produce progeny of SSCs.
Materials and Mehtods: Testes from 3, 5 and 7 months old calves were utilized to examine isolation, purity and in –vitro propogation of SSCs. Cells were isolated and purified with two-stemp enzymatic digestion combined with centrifugal separation on discontinuous Percoll density gradient layers.
Findings: Cell propagation and SSCs marker expression were determined at 5, 10 and 15 days post-culture.  Immunostaining in conjunction with transcrioption based marker expression of cultured cells showed that stem cell markers (DBA, UCHL1, Oct4 and Sox2) were expressed in SSCs.
Conclusion: The  results demonstrated marker expression of bull spermatogonial stem cells and showed that prior to transplantation in-vitro culturing of bull SSCs is implementable.

Keywords

Bovine, In-vitro propogation, Pre-pubertal, SSCs, Testis

##plugins.themes.academic_pro.article.details##

How to Cite
Wardak, M. K., Khalid, A. U. R., & Jameel, Z. (2023). In-vitro propagation of pre-pubertal bovine Spermatogonial Stem Cell in prepation for transplantation. Nangarhar University International Journal of Biosciences, 2(01), 15–25. https://doi.org/10.70436/nuijb.v2i01.17

References

  1. Ahmad, S., Xiao, Y., Han, L., Hua, H., Riaz, H., Liang, A., & Yang, L. G. (2013). Isolation, identification and enrichment of type a spermatogonia from the testis of chinese cross-bred buffaloes (Swamp×River). Reproduction in Domestic Animals, 48(3), 373–381.
  2. Aponte, P. M., Soda, T., van de Kant, H. J. G., & de Rooij, D. G. (2006). Basic features of bovine spermatogonial culture and effects of glial cell line-derived neurotrophic factor. Theriogenology, 65(9), 1828–1847.
  3. Aponte, P. M., Soda, T., Treeds, K., Mizark, S. C., van de Kan, H. J. G., & de Rooji, D. G. (2008) Propagation of bovine spermatogonial stem cells in vitro. Reproduction, 136(5), 543-557.
  4. Aponte, P. M., Van Bragt, M. P., De Rooij, D. G., & Van Pelt, A. M. (2005). Spermatogonial stem cells: characteristics and experimental possibilities. Apmis, 113(11‐12), 727-742.
  5. Bellve, A. R., Cavicchia, J. C., Millette, C. F., O'Brien, D. A., Bhatnagar, Y. M., & Dym, M. (1977). Spermatogenic cells of the prepuberal mouse: isolation and morphological characterization. The Journal of cell biology, 74(1), 68-85.
  6. Brinster, R. L., & Zimmermann, J. W. (1994). Spermatogenesis following male germ-cell transplantation. Proceedings of the National Academy of Sciences, 91(24), 11298-11302.
  7. Conrad, S., Renninger, M., Hennenlotter, J., Wiesner, T., Just, L., Bonin, M., & Skutella, T. (2008). Generation of pluripotent stem cells from adult human testis. Nature, 456(7220), 344-349.
  8. Deluz, C., Friman, E. T., Strebinger, D., Benke, A., Raccaud, M., Callegari, A., & Suter, D. M. (2016). A role for mitotic bookmarking of SOX2 in pluripotency and differentiation. Genes & development, 30(22), 2538-2550.
  9. Dobrinski, I., & Travis, A. J. (2007). Germ cell transplantation for the propagation of companion animals, non-domestic and endangered species. Reproduction, Fertility and Development, 19(6), 732–739.
  10. Fujihara, M., Kim, S.-M., Minami, N., Yamada, M., & Imai, H. (2011). Characterization and in vitro culture of male germ cells from developing bovine testis. The Journal of Reproduction and Development, 57(3).
  11. Goel, S., Fujihara, M., Minami, N., Yamada, M., & Imai, H. (2008). Expression of NANOG, but not POU5F1, points to the stem cell potential of primitive germ cells in neonatal pig testis. Reproduction, 135(6), 785.
  12. Goel, S., Reddy, N., Mandal, S., Fujihara, M., Kim, S. M., & Imai, H. (2010). Spermatogonia-specific proteins expressed in prepubertal buffalo (Bubalus bubalis) testis and their utilization for isolation and in vitro cultivation of spermatogonia. Theriogenology, 74(7), 1221-1232.
  13. Hagey, D. W., Klum, S., Kurtsdotter, I., Zaouter, C., Topcic, D., Andersson, O., ... & Muhr, J. (2018). SOX2 regulates common and specific stem cell features in the CNS and endoderm derived organs. PLoS genetics, 14(2), e1007224.
  14. He, Y., Chen, X., Zhu, H., & Wang, D. (2015). Developments in techniques for the isolation, enrichment, main culture conditions and identification of spermatogonial stem cells. Cytotechnology, 67(6), 921–930.
  15. Heidari, B., Rahmati-Ahmadabadi, M., Akhondi, M. M., Zarnani, A. H., Jeddi-Tehrani, M., Shirazi, A., Naderi, M. M., & Behzadi, B. (2012). Isolation, identification, and culture of goat spermatogonial stem cells using c-kit and PGP9.5 markers. Journal of Assisted Reproduction and Genetics, 29(10), 1029–1038.
  16. Herrid, M., Davey, R. J., & Hill, J. R. (2007). Characterization of germ cells from pre-pubertal bull calves in preparation for germ cell transplantation. Cell and Tissue Research, 330(2), 321–329.
  17. Izadyar, F., Den Ouden, K., Creemers, L. B., Posthuma, G., Parvinen, M., & De Rooij, D. G. (2003). Proliferation and differentiation of bovine type A spermatogonia during long-term culture. Biology of Reproduction, 68(1), 272–281.
  18. Izadyar, F., Spierenberg, G. T., Creemers, L. B., den Ouden, K., & de Rooij, D. G. (2002). Isolation and purification of type A spermatogonia from the bovine testis. Reproduction, 124(1), 85–94.
  19. Kaavya, R., Kannan, T., Basha, S., Vairamuthu, S., Ramesh, G., & William, B. (2016). Isolation of Spermatogonial Stem Cell and Its Viability in Mice by Different Enzymatic Digestion Methods. International Journal of Livestock Research, 6(11), 54–60.
  20. Kanatsu-Shinohara, M., Miki, H., Inoue, K., Ogonuki, N., Toyokuni, S., Ogura, A., & Shinohara, T. (2005). Long-Term Culture of Mouse Male Germline Stem Cells Under Serum-or Feeder-Free Conditions1. Biology of Reproduction, 72(4), 985–991.
  21. Kanatsu-Shinohara, M., Ogonuki, N., Inoue, K., Miki, H., Ogura, A., Toyokuni, S., & Shinohara, T. (2003). Long-Term Proliferation in Culture and Germline Transmission of Mouse Male Germline Stem Cells. Biology of Reproduction, 69(2), 612–616.
  22. Kanatsu-Shinohara, M., Toyokuni, S., & Shinohara, T. (2004). Transgenic mice produced by retroviral transduction of male germ line stem cells in vivo. Biology of Reproduction, 71(4), 1202–1207.
  23. Khaira, H., µl, D., Ohl, D. A., & Smith, G. D. (2005). Spermatogonial stem cell isolation, storage, and transplantation. Journal of Andrology, 26(4), 442–450.
  24. Kim, S. M., Fujihara, M., Sahare, M., Minami, N., Yamada, M., & Imai, H. (2014). Effects of extracellular matrices and lectin Dolichos biflorus agglutinin on cell adhesion and self-renewal of bovine gonocytes cultured in vitro. Reproduction, Fertility and Development, 26(2), 268–281.
  25. Kim, Y., Turner, D., Nelson, J., Dobrinski, I., McEntee, M., & Travis, A. J. (2008). Production of donor-derived sperm after spermatogonial stem cell transplantation in the dog. Reproduction, 136(6), 823–831.
  26. Liu, S., Tang, Z., Xiong, T., & Tang, W. (2011). Isolation and characterization of human spermatogonial stem cells. Reproductive Biology and Endocrinology, 9(1), 141.
  27. Lou, J., Megee, S., & Dobrinski, I. (2009). Asymmetric distribution of UCH-L1 in spermatogonia is associated with maintenance and differentiation of spermatogonial stem cells. Journal of Cellular Physiology, 220(2), 460–468.
  28. Manku, G., & Culty, M. (2015). Mammalian gonocyte and spermatogonia differentiation: Recent advances and remaining challenges. Reproduction, 149(3), R139–R157.
  29. McLean, D. J., Johnston, D. S., Russell, L. D., & Griswold, M. D. (2001). Germ cell transplantation and the study of testicular function. Trends Endocrinol Metab, 12(1), 16–21.
  30. Oatley, J. M., & Brinster, R. L. (2008). Regulation of spermatogonial stem cell self-renewal in mammals. Annual review of cell and developmental biology, 24, 263-286.
  31. Oatley, J. M., Reeves, J. J., & McLean, D. J. (2004). Biological activity of cryopreserved bovine spermatogonial stem cells during in vitro culture. Biology of Reproduction, 71(3), 942–947.
  32. Olive, V., Cuzin, F., Hogarth, C. A., Griswold, M. D. M., Sadri-ardekani, H., Atala, A., Meistrich, M. L. L., Van Beek, M., Khaira, H., McLean, D. J., Ohl, D. A., Smith, G. D., Phillips, B. T., Gassei, K., Orwig, K. E., Dym, M., Kokkinaki, M., He, Z., Zheng, Y., … Tournaye, H. (2015). Spermatogonial stem cell regulation and spermatogenesis. Molecular and Cellular Endocrinology, 419(3), 1663–1678.
  33. Pan, G. U. J. in, Chang, Z., Er, H. ansrsh, & Pei, D. U. (2002). Stem cell pluripotency and transcription factor Oct4. Cell Research, 12(5–6), 321–329.
  34. Phillips, B. T., Gassei, K., & Orwig, K. E. (2010). Spermatogonial stem cell regulation and spermatogenesis. Philosophical Transactions of the Royal Society B: Biological Sciences, 365(1546), 1663-1678.
  35. Reding, S. C., Stepnoski, A. L., Cloninger, E. W., & Oatley, J. M. (2010). THY1 is a conserved marker of undifferentiated spermatogonia in the pre-pubertal bull testis. Reproduction, 139(5), 893-903.
  36. Rodriguez-Sosa, J. R., Dobson, H., & Hahnel, A. (2006). Isolation and transplantation of spermatogonia in sheep. Theriogenology, 66(9), 2091-2103.
  37. Aponte, P. M., Soda, T., Teerds, K. J., Mizrak, S. C., van de Kant, H. J., & de Rooij, D. G. (2008). Propagation of bovine spermatogonial stem cells in vitro. Reproduction, 136(5), 543.
  38. Ryu, B. Y., Orwig, K. E., Kubota, H., Avarbock, M. R., & Brinster, R. L. (2004). Phenotypic and functional characteristics of spermatogonial stem cells in rats. Developmental biology, 274(1), 158-170.
  39. Schlatt, S. (2002). Spermatogonial stem cell preservation and transplantation. Molecular and cellular endocrinology, 187(1-2), 107-111.
  40. Schulz, R. W., & Miura, T. (2002). Spermatogenesis and its endocrine regulation. Fish physiology and biochemistry, 26, 43-56.
  41. Seandel, M., Daylon, J., Shemlkov, S. V., Falciatori, I., Jiyeon, K., Chavala, S., Scherr, D. S., Fan, Z., Torres, R., Gale, N. W., Yancopoulos, G. D., Murphy, A., David M., V., Hobbs, R. M., Pandolfi, P. P., & Rafil, S. (2007). Generation of functional multipotent adult stem cells from GPR125+ germline progenitors. Nature, 449(7160), 346–350.
  42. Shafiei, S., Tajik, P., Nava, G., Garoussi, T., Panahi, Q., & Feyli, R. (2013). Isolation of bovine spermatogonial cells and coculture with prepubertal sertoli cells in the presence of colony stimulating factor-1 Key words : Abstract : Iranian Jounal of Veterinary Medicine, 7(2), 83–90.
  43. Shi, G., & Jin, Y. (2010). Role of Oct4 in maintaining and regaining stem cell pluripotency. Stem Cell Research and Therapy, 1(5), 1–9.
  44. Vansandt, L. M. (2014). Characterization, enrichment, and in vitro culture of spermatogonial stem cells in the domestic cat: A model for rare and endangered felids (Doctoral dissertation, University of Maryland, College Park).
  45. Wahab-Wahlgren, A., Martinelle, N., Holst, M., Jahnukainen, K., Parvinen, M., & Söder, O. (2003). EGF stimulates rat spermatogonial DNA synthesis in seminiferous tubule segments in vitro. Molecular and Cellular Endocrinology, 201(1-2), 39-46.
  46. Wrobel, K. H. (2000). Prespermatogenesis and spermatogoniogenesis in the bovine testis. Brain Structure and Function, 202(3), 209.
  47. Yamaguchi, S., Kimura, H., Tada, M., Nakatsuji, N., & Tada, T. (2005). Nanog expression in mouse germ cell development. Gene Expression Patterns, 5(5), 639–646.
  48. Zhang, R., Sun, J., & Zou, K. (2016). Advances in Isolation Methods for Spermatogonial Stem Cells. Stem Cell Reviews and Reports, 12(1), 15–25.
  49. Zhang, S., & Cui, W. (2014). Sox2, a key factor in the regulation of pluripotency and neural differentiation. World Journal of Stem Cells, 6(3), 305.
  50. Zhao, H. M., Yang, H., Luo, F. H., Li, M. X., Zhang, S., & Yang, X. G. (2016). Isolation , proliferation , and induction of Bama mini-pig spermatogonial stem cells in vitro. Genetics and Molecular Research, 15(3), 1–13.
  51. Zheng, Y., Zhang, Y., Qu, R., He, Y., Tian, X., & Zeng, W. (2014). Spermatogonial stem cells from domestic animals: Progress and prospects. Reproduction, 147(3), 65–74.

Most read articles by the same author(s)