In-vitro propagation of pre-pubertal bovine Spermatogonial Stem Cell in prepation for transplantation

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Published Feb 25, 2023
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Vol. 2 No. 01 (2023): February

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Mohammad Kazim Wardak
Aziz Ur Rahman Khalid
Zabihullah Jameel

Abstract

‌Backgroud: We investigated the expression of stem cell markers in in -vitro cultivated speramatogonial stem cells (SSCs) with the intention of developing a useful system to produce progeny of SSCs.
Materials and Mehtods: Testes from 3, 5 and 7 months old calves were utilized to examine isolation, purity and in –vitro propogation of SSCs. Cells were isolated and purified with two-stemp enzymatic digestion combined with centrifugal separation on discontinuous Percoll density gradient layers.
Findings: Cell propagation and SSCs marker expression were determined at 5, 10 and 15 days post-culture.  Immunostaining in conjunction with transcrioption based marker expression of cultured cells showed that stem cell markers (DBA, UCHL1, Oct4 and Sox2) were expressed in SSCs.
Conclusion: The  results demonstrated marker expression of bull spermatogonial stem cells and showed that prior to transplantation in-vitro culturing of bull SSCs is implementable.

Keywords

Bovine, In-vitro propogation, Pre-pubertal, SSCs, Testis

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How to Cite
Wardak, M. K., Khalid, A. U. R., & Jameel, Z. (2023). In-vitro propagation of pre-pubertal bovine Spermatogonial Stem Cell in prepation for transplantation. NUIJB, 2(01), 15–25. Retrieved from https://nuijb.nu.edu.af/index.php/nuijb/article/view/17

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