Amelioration of Heat Stress-Induced Alterations in Immune indices, Serum Enzyme Activity, Antioxidant Ability and Gene Expression in Wistar Rats through Nutritional Strategies

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Published Feb 10, 2024
https://doi.org/10.70436/nuijb.v3i02.179
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2024: ICCC (Special Issue)

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Mohammad Malyar Rahmani
Maazullah Nasim
Abdul Waris Shinwari
Mohammad Bayer Darmel

Abstract

High ambient temperature and humidity have a negative impact on livestock health and can lead to heat stress in animals. The objective of this study was to assess the impacts of SeP, ZnP, P and SeZnP on the growth performance, serum enzyme activity, antioxidant status, immune function, and mRNA expression of Wistar rats exposed to high ambient temperature. Seventy-two male Wistar rats were randomly allocated to six groups: Control (CON), high temperature (HT), high temperature plus probiotics (HT+P, CFU, L. acidophilus 1011/mL and S. cerevisiae 109/mL), high temperature plus zinc-fortified probiotics (HT+ZnP, 100mg/L), high temperature plus selenium-fortified probiotics (HT+SeP, 0.3mg/L), and high temperature plus selenium/zinc-fortified probiotics (HT+SeZnP, 0.3mg/L + 100mg/L). The feeding period lasted forty days, and blood and tissue samples were collected on the final day. The results demonstrated that all supplemented groups (P, SeP, ZnP, and SeZnP) significantly enhanced growth performance compared to the control group (p < 0.05). Notably, SeZnP supplementation significantly increased GSH content, SOD activity, GSH-Px activity, while reduced MDA content, creatinine, LDH, CK, ALP, AST, ALT, and blood urea nitrogen levels in the serum of Wistar rats. The concentrations of IFN-γ, IL-6, and IL-2 (p < 0.05) increased with all supplemented treatments, while IL-10 decreased. Moreover, SeP, ZnP, and SeZnP significantly upregulated the expression of GPx1 and SOD1 genes (p < 0.05), while downregulated Hsp90 and Hsp70 heat shock genes (p < 0.05). In conclusion, this product shows potential as a nutritive supplement for animals exposed to high ambient temperatures. Implementing this strategy can help producers maintain the health, comfort, and productivity of their animals during the summer season.

Keywords

Antioxidant, status, Genes, expression, Heat, stress, Immune, function, Nutritional, Strategies, Wistar, rats

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How to Cite
Rahmani, M. M., Nasim, M., Shinwari, A. W., & Darmel, M. B. (2024). Amelioration of Heat Stress-Induced Alterations in Immune indices, Serum Enzyme Activity, Antioxidant Ability and Gene Expression in Wistar Rats through Nutritional Strategies. Nangarhar University International Journal of Biosciences, 3(02), 113–118. https://doi.org/10.70436/nuijb.v3i02.179

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